The Selective Use of Bioabsorbable Plates and Screws According to Fracture Site and Aspect of Mandible

Currently, fixation with "absorbable plates and screws" is widely used for the treatment of facial bone fracture. But it is carefully used for mandible fracture, because it needs much stronger skeletal stability caused by deviation in part of fracture. And there are some complications by using it. We need "absorbable plates and screws" depending on the case of fracture site of mandible and combined fracture. And it was classified favorable or unfavorable fracture according to the aspect of fracture site. In favorable fracture without deviation of symphysis, only "one absorbable plate and screws" has been used, and in unfavorable fracture, we used "two absorbable plates and screws". In the cases of body and angle fracture, "two absorbable plates and screws" have been used because there is more underneath load in body and angle fracture than in symphysis fracture. As this way, we basically used Biosorb FX(R) 2.0 system and applied 21 patients in 34 cases. Finally, we got enough skeletal stability in mandibular fracture and there were not any complications such as malocclusion, ununion and late tissue response. So we report these results along with document consideration.

Application of Human Dermal Fibroblast and Keratinocyte on Allogenic Dermis(AlloDerm(R))

PURPOSE: Large skin defect by various causes, should be covered by autologous skin graft. But, the donor site of autologous skin graft is limited and leaves permanent donor scar and contracture. There have been our trial to engineer artificial skin using allogenic dermis (AlloDerm) with basement membrane. METHODS: Dermal and epidermal layer were separated by immersing in dipase solution for 30 minutes, and the separated layers were treated with 0.05% trypsin for 10 minutes. And then each layer was cultivated to fibroblasts and keratinocytes on a culture medium. Fibroblasts were first penetrated into basement membrane of allogenic dermis facing down, then allogenic dermis was flipped over to face up and keratinocytes were transplanted to allogenic dermis. RESULTS: Observing artificial skin fabricated in vitro, we found following: 1) The artificial skin opened in air for 5 days formed epidermal layer. In dermal layer, fibroblast was distributed evenly among all. 2) The artificial skin opened in air for 30 days formed thicker and thicker, and it formed basement membrane, spinous and granular layers. PAS stain to confirm existence of basement membrane showed positive reaction. 3) Cytokeratin 10 stain to confirm the formation of epidermal layer showed positive reaction. 4) The formation of thick keratin, lamellar body and desmosome similar to human skin were observed in result of an electron micrograph. CONCLUSION: As a result of research, the structure seen in normal skin such as rete ridge, is found in reproduced artificial skin. This type of artificial skin can be used as a useful model for investigating skin disease and for clinical application also.